Extended Data Figure 1: Effect of miR-25 on IP3R1 and cardiomyocyte calcium transients in vitro.
From: Inhibition of miR-25 improves cardiac contractility in the failing heart
a, Effect of miR-25, anti-miR-25 and siIP3R1 transfection on IP3R1 protein levels in HL-1 cells. b, Sequence of the putative miR-25 target recognition element in IP3R1 mRNA and the corresponding alteration by site-directed mutagenesis. Mutation abolished inhibition of the luciferase signal by miR-25 (n = 10). a, b, Data are represented as mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001. NS, not significant (Student’s t-test). c, Representative Ca2+ transient of HL-1 cells transfected as indicated. Note that miR-25 slowed the repolarization phase kinetics, whereas anti-miR-25 quickened the kinetics. Co-transfection normalized kinetics. d, Kinetic imaging cytometry analysis of Ca2+ transient kinetics during the decay phase (Ca2+ transient duration time from 75% to 25% maximal value, CaTD75–25) of transfected NRVCs (n > 550 cells). Box defines interquartile range; bar shows median; whiskers show ± 5th and 95th percentile; dots indicate outliers. *P < 0.05, **P < 0.01, ***P < 0.001. NS, not significant (one-tailed ANOVA). Experiments were replicated two times.
