Extended Data Figure 4: Effect of miR-25 and miR-92a overexpression in vivo.
From: Inhibition of miR-25 improves cardiac contractility in the failing heart
a, Effect of AAV9-mediated cardiac gene transfer of miR-25 and miR-92a on target protein levels. Cardiac gene transfer of miR-25 and miR-92a increased levels of their respective miRNAs but miR-92a was selective against confirmed target integrin subunit α5 (ITGA5). b, c, Effects of AAV9-mediated cardiac gene transfer of miR-25 and miR-92a on cardiac function. There is a tendency towards decreased cardiac function in both ESPVR (b) and ΔP/Δtmax (c) in AAV9-miR-25-treated animals relative to control- (AAV9-VLP) and AAV9-miR-92a-treated animals. n = number of biological replicates (all included): n = 5 (AAV9-VLP); 4 (AAV9-miR-25); 5 (AAV9-miR-92a) for panels b, c. Data are represented as mean ± s.e.m. NS, not significant.
