Extended Data Figure 6: High-molecular-weight lignin polymers are underrepresented in med5a/5b ref8-1 mutants.
From: Disruption of Mediator rescues the stunted growth of a lignin-deficient Arabidopsis mutant
Shown are the results of gel-permeation chromatography of lignin from wild-type, med5a/5b and med5a/5b ref8-1 cell walls. The x-axis indicates the apparent molecular weight of individual lignin polymer fragments and is shown as a log scale. The y-axis shows the response of an ultraviolet-light detector normalized to the most abundant signal in each chromatogram. The most abundant signal in all samples corresponds to a molecular weight of ∼10,000 Da, whereas a secondary peak at ∼250,000 Da is significantly underrepresented in lignin derived from the med5a/5b ref8-1 mutant.
