Extended Data Figure 3: Localization of membrane transporters identified in the endo-lysosome fraction.
From: Endosomes are specialized platforms for bacterial sensing and NOD2 signalling
a, Membrane transporters identified by mass spectrometry were expressed as GFP fusion proteins in 293 cells, then observed by fluorescence microscopy. Endo-lysosomes are visualized with ovalbumin–Alexa555 (OVA) or dextran–Alexa555. Single arrows indicate colocalization of membrane transporters labelled with endocytosed OVA or dextran. Tubular endo-lysosomes were observed only in cells expressing SLC15A3 or SLC15A4 (arrow heads). Double arrows on cells expressing SLC7A11, ABCC4 or ABCC1 mark plasma membrane. b, SLC15A1 (mouse) or SLC15A2 (human) were expressed as GFP fusion proteins in 293 cells, then observed by fluorescence microscopy. Arrows mark plasma membrane. Bars, 5 µm. c, Gene expression of SLC15A1 and SLC15A2 were quantified by RT–PCR. Means ± s.d. (+) denotes LPS-stimulated dendritic cells.
