Extended Data Figure 3: Optogenetic stimulation of the imaged area evokes forelimb movements in awake mice.
From: Emergence of reproducible spatiotemporal activity during motor learning
a, Optogenetic excitation of the imaged area triggers forelimb movements in mice expressing ChR2 but not in control mice not expressing ChR2 (P < 0.001, chi-squared test). ChR2 expression does not alter spontaneous movement frequency in the absence of stimulation (P = 0.64, chi-squared test). ‘During light’, 1-s light stimulation; ‘before light’, 2 to 1 s before light onset, n = 40 ‘during light’ trials and 38 ‘before light’ trials in two ChR2 mice, 38 ‘during light’ trials and 38 ‘before light’ trials in two control mice. b, Histological section showing the expression of ChR2 in the motor cortex. Green, ChR2–YFP; blue, DAPI.
