Extended Data Figure 4: Simultaneous cell-attached recordings and two-photon calcium imaging in awake mice.
From: Emergence of reproducible spatiotemporal activity during motor learning
a, Left: in vivo two-photon image of motor cortex neurons expressing GCaMP5G. The neuron in the centre is targeted with a patch electrode. Right: after the recording session, voltage step was applied to the electrode to activate the recorded neuron. The increased GCaMP5G fluorescence in the middle neuron confirms that the neuron was indeed targeted. b, Example GCaMP5G fluorescence trace (top: black indicates fluorescence trace and red indicates detected calcium events) and simultaneously recorded action potentials (bottom: black vertical ticks; the numbers indicate the number of action potentials contained in each burst). Horizontal red lines at bottom indicate the duration of detected calcium events. Note the precise temporal relationship between action potentials and calcium events. c, Table summarizing data from six neurons in two mice. Positive offsets indicate the lag of the onset of detected calcium events relative to the first spike in the burst. The offset (7.1 ± 41.4 ms) is on the order of the temporal resolution of our imaging (∼35 ms per image frame).
