Extended Data Figure 4: UVR-accelerated BRAF(V600E)-driven tumours have similar histology to BRAF(V600E)-driven tumours from non-UVR-exposed animals.
From: Ultraviolet radiation accelerates BRAF-driven melanomagenesis by targeting TP53
a, Photomicrograph of an H&E-stained tumour from a UVR-treated BRAF(V600E) mouse highlighting the presence of atypical heterogeneous spindle dendritic and pigment-producing cells (black asterisks and arrow respectively, left panel) and atypical plump spindle cells and mitotic figures (white asterisks and black arrowhead respectively, right panel). Scale bar, 25 µm. b, Photomicrograph of an H&E-stained tumour from a UVR-treated BRAF(V600E) mouse highlighting a region of dermal ulceration (black arrow). Scale bar, 0.5 mm. c, Photomicrograph of H&E-stained tumours from non-UVR exposed (left panel) and UVR-exposed (right panel) BRAF(V600E) mice, showing the presence of nuclear pleomorphism (asterisks) in both tumours. Scale bar, 10 µm. d, Photomicrograph of an S100-stained tumour from a UVR-exposed BRAF(V600E) mouse. Scale bar, 100 µm. e, Photomicrograph of an HMB-45/MelanA-stained tumour from a UVR-exposed BRAF(V600E) mouse. Scale bar, 50 µm. f, Photomicrograph of a Ki-67-stained tumour from a UVR-exposed BRAF(V600E) mouse. Scale bar, 50 µm.
