Extended Data Figure 6: Cleavage of Masc mRNA.
From: A single female-specific piRNA is the primary determiner of sex in the silkworm
a, Identification of the cleavage site of Masc mRNA. The Masc mRNA-derived RNA fragments were amplified by a modified RACE method, cloned, and sequenced. The RACE adaptor and the cloned 5′-end are indicated. Thirteen 5′-ends were determined and all showed identical sequences. Nucleotides identical to the top sequence are represented by asterisks. b, Detection of Masc piRNA. Northern blot analysis was performed using total RNA prepared from early embryos. The asterisk shows the location of Masc piRNA. c, Mapping of embryonic piRNAs (24 hpo) onto Masc mRNA. The relative location of ORF of Masc is shown below. d, Normalized reads of Fem piRNA and Masc piRNA in Siwi- or BmAgo3-immunoprecipitated libraries from BmN4 cells14. e, A ping-pong amplification model of Fem piRNA/Masc piRNA. f, Normalized reads of Masc piRNA in embryonic piRNA libraries. Reads of 26–29 nucleotides that showed 2 or fewer mismatches to the Masc piRNA sequence were scored as positive. g, RT–qPCR estimation of Masc piRNA in early embryos. The Masc piRNA level was normalized to that of let-7.
