Extended Data Figure 7: Effects of Siwi or BmAgo3 knockdown on the Bmdsx splicing and Masc expression.
From: A single female-specific piRNA is the primary determiner of sex in the silkworm
a, Masc expression in female embryos injected with two types of siRNAs that target Siwi (Siwi-1 and Siwi-2) or a control siRNA that targets GFP. Total RNA was isolated from female siRNA-injected embryos at 18 hpo and RT–qPCR was performed. The data shown are mean + s.d. The number at the base of each bar indicates the sample size of each group. Data were subjected to Kruskal–Wallis analysis with post hoc Dunn’s test. *P < 0.05. The expression levels of Siwi mRNA decreased to 23 and 44% after injecting Siwi-1 and Siwi-2 siRNAs, respectively, compared with that in GFP-siRNA-injected embryos. b, Knockdown of BmAgo3 mRNA in newly hatched larvae. The embryos were injected with BmAgo3 or GFP (control) siRNA. Total RNA was isolated from newly hatched larvae (at about 240 h post-injection) and RT–qPCR was performed. The data shown are mean + s.d. The number indicates the sample size of each group. *P < 0.05, one-sided Mann–Whitney test. c, Splicing of Bmdsx in newly hatched larvae that were injected with BmAgo3 siRNA. The Bmdsx splicing patterns were examined at about 240 h post-injection. The number indicates the sample size. The abbreviations are the same as in Fig. 2c. d, Masc expression in newly hatched larvae that were injected with BmAgo3 siRNA. Total RNA was isolated from siRNA-injected newly hatched larvae (at about 240 h post-injection) and RT–qPCR was performed. The data shown are mean + s.d. The number indicates the sample size of each group. *P < 0.05, one-sided Mann–Whitney test.
