Extended Data Figure 1: Molecular sexing and comparative transcriptome analysis of embryonic B. mori.
From: A single female-specific piRNA is the primary determiner of sex in the silkworm
a, Molecular sexing of individual embryos at 21 hpo. Musashi, Sasuke and Bonsai are W chromosome RAPD markers. ‘Chr2’ control bands are generated from a primer set that amplifies a sequence within the 2nd chromosome of B. mori. b, MA plots of RNA-seq data. The comp73859_c0 contig is indicated by red dots and highlighted by arrows. The axes show: A (x-axis) = (log2(transcripts per million in male) + log2(transcripts per million in female))/2. M (y-axis) = log2(transcripts per million in male) − log2(transcripts per million in female). c, Number of the comp73859_c0-derived transcripts in each RNA-seq library. Note that the comp73859_c0-derived transcripts detected in male libraries may be derived from incorrectly sexed embryos or RNA produced by polar bodies. Combined with RT–qPCR results of Fig. 1c, the expression level of this contig peaks around 18–21 hpo in the B. mori embryo.
