Extended Data Figure 6: Quantitative information from RNA-seq data.
From: WNT7A and PAX6 define corneal epithelium homeostasis and pathogenesis
a, Statistical analysis of RNA-seq samples: raw reads, mapping reads and mapping rate of each sample are included. b, Pairwise comparisions of duplicated biological samples. c, The differences between SECs and three-dimensional PAX6+ SESCs, CECs and three-dimensional shPAX6 LSCs, all FDR < 0.001. a, qPCR analysis of PAX6 expression in rabbit SESCs with PAX6 transduction (rabbit (Rb) PAX6+ SESCs) or LSCs with PAX6 knockdown (Rb shPAX6 LSCs) (all n = 3, P < 0.05). We noticed some minor differences in the heatmap. These might result from some experimental variations, or it is possible that, although PAX6 expression is largely responsible for cell fate switch from SESCs to CECs at gene expression and functional levels (as demonstrated in this study), this single transcription factor may not be sufficient to create cells that are completely identical to CECs.
