Extended Data Figure 8: Ectopic overexpression of Ndr did not induce ectopic bud formation.
From: Nodal signalling determines biradial asymmetry in Hydra
a, Budless animals were electroporated with pBSAA-GFP (control) or pBSAA-Ndr vectors. After a 2-day incubation in hydra medium, cells expressing GFP or H. magnipapillata Ndr or Pitx genes were visualized by WISH. Ectopic overexpression of Ndr resulted in a slight increase in Pitx expression along the main body axis, but a new head organizer with strong Pitx expression was established only in the budding zone. b, Quantification of transfected cells demonstrated an almost equal distribution of GFP- or Ndr-expressing cells along the oral–aboral axis. This finding indicates that the whole body column region has a similar capacity to be transfected with an Ndr-expressing plasmid and to express an exogenous Ndr gene.
