Extended Data Figure 2: The cryo-EM map of the 39S subunit within the 55S mitoribosome.
From: The complete structure of the large subunit of the mammalian mitochondrial ribosome
a, Surface rendering of the 39S subunit cryo-EM map at 3.4 Å resolution, viewed from the solvent side. Landmarks are indicated: CP, central protuberance; L1, L1 stalk; L7/L12, L7/L12 stalk base; TE, polypeptide tunnel exit region. b, Black: FSC curve of the 39S cryo-EM reconstruction. The resolution is 3.4 Å according to the FSC = 0.143 criterion53. Curve computed using RELION9 from auto-masked maps computed from data half-sets. Red: the FSC curve computed between the final reconstruction and the refined coordinates (see Extended Data Fig. 3) indicates a resolution of 3.4 Å according to the FSC = 0.5 criterion, which has to be used in this case53. c, Local resolution plot. d–g, Examples for the quality of the density: an rRNA double helix (d), a base pair (e), interactions of protein and rRNA with many large side chains visible (f), and a Mg2+ ion coordinated by the rRNA (g).
