Extended Data Figure 4: CSPG and ISP regulation of Erk1/2.
From: Modulation of the proteoglycan receptor PTPσ promotes recovery after spinal cord injury
Western blot analysis revealed a significant decrease in the phosphorylation ratio of Erk1/2 in SH-SY5Y cells plated on laminin (2 μg ml−1) plus CSPGs (15 μg ml−1) compared with laminin-only substrates, which was reversed by either pre-treatment with ChABC (0.1 U ml−1) or 4 days of ISP treatment (2.5 μM). pErk1/2, phosphorylated Erk1/2; tErk1/2, total Erk1/2. Data normalized to laminin control. N = 4 independent experiments. *P < 0.05 versus all other conditions, one-way ANOVA, Tukey’s post-hoc test.
