Extended Data Figure 6: Analysis of DSB interference across the ARE1 region.

a, DSB interference was calculated in sae2Δ (top) and sae2Δ tel1Δ (bottom) using the following formula: 1–f(observed double-cuts)/f(expected double-cuts), where the expected double-cut values were calculated using two methods. Left, single-cut frequencies were measured by Southern-blot using a TAF2 probe (for DSB sites on the left of ARE1) or a PWP2 and RSC6 probe (for the right-hand side of ARE1; Extended Data Table 2). Right, calculations were made after converting the measured Spo11-oligo frequency¹ at each DSB site to a % DSB ± s.d. value by using the measured DSB frequency at ARE1 in sae2Δ or sae2Δ tel1Δ for normalization (see Notes below table and Methods for further details). b, Chart of observed (column B) and expected (column F and Q) frequencies of double-cuts. Error bars, s.d. n = 2. P values, two-tailed t-test. Double-cut products that were present at a frequency that was statistically different from that for no interference (independence) were highlighted in a according to the type of interference present: red indicates positive DSB interference, blue indicates negative DSB interference (concerted DSB formation); in b the same statistical differences were indicated with open diamonds or asterisks, respectively.