Extended Data Figure 9: Controls for the regulation of RP gene expression experiments.
From: NIK1-mediated translation suppression functions as a plant antiviral immunity mechanism
a–i, Expression of the indicated genes in the leaves of independent transgenic lines was monitored by qRT–PCR. a, LIMYB expression in the limyb-32 mutant was examined. b, LIMYB expression in limyb-32 mutant restores wild-type expression of the RP genes. Expression of the S13a, L18A and L28e genes was monitored in three independently transformed limyb-32 knockout plants with the LIMYB gene. c–f, Expression of the unrelated gene AtWWP1 was monitored as a negative control in three independently transformed LIMYB-, RPL10- and T474D-overexpressing lines in addition to the limyb-32 mutant. g, The double-mutant inactive kinase, G4743V/T474A, does not downregulate the RP genes. The transcript accumulation of the indicated RP genes was quantified by qRT–PCR in two independently transformed nik1 knockout lines expressing the G4743V/T474A double mutant. h, i, Expression of LIMYB (h) and the transgene T474D (i) was monitored in the limyb-32 lines, which were transformed with T474D. a–i, Means ± 95% confidence intervals (n = 3) based on bootstrap resampling replicates of three independent experiments are shown.
