Extended Data Figure 2: Expression of immune-system-related genes in T474D-overexpressing lines and in LIMYB-overexpressing lines.
From: NIK1-mediated translation suppression functions as a plant antiviral immunity mechanism
a–c, On the basis of our global comparison of EST sequences (Fig. 1a) and the role of NIK as an antiviral receptor, we asked whether the constitutive activation of NIK would elicit a defence response similar to that induced by geminivirus infection via the salicylic acid pathway or typical defence responses to virus. No significant gene enrichment was detected in the virus-induced gene silencing (GO:0009616) and viral defence response (GO:0051607) categories using the gene set enrichment analysis (GSEA) method (Supplementary Table 2). For the immune system category, gene enrichment was found in both up- and downregulated changes using the GSEA method. However, the typical markers of salicylic acid signalling, such as PR1 and SNC1, were either non-differentially expressed or downregulated, and the expression of T474D did not enhance the salicylic acid level in the transgenic lines. Collectively, these results indicate that ectopic expression of T474D did not activate typical viral defences, such as salicylic acid signalling or gene silencing. a, b, Transcript accumulation of selected immune-system-related gene markers by RT–PCR (a) or RNA-sequencing in T474D-overexpressing lines (b). qRT–PCR of a representative sample confirmed an 80% match with the RNA-sequencing results. c, Transcript accumulation of the immune-system-related genes in LIMYB-overexpressing lines. The expression of the indicated genes in the leaves of independent transgenic lines was monitored by qRT–PCR. a, c, Mean ± 95% confidence intervals (n = 3) are shown, based on bootstrap resampling replicates of three independent experiments.
