Extended Data Figure 10: Dietary C18:0 improves Parkinson’s disease phenotypes of Pink and Parkin mutant flies.
From: Regulation of mitochondrial morphology and function by stearoylation of TFR1
a, b, Dietary C18:0 supplementation (10%) significantly increases lifespan of male Parkin25 (a) and Pink1B9 (b) mutant flies. n = 8 × 10 animals. c, Dietary C18:0 supplementation rescues ATP levels of 1-week-old male Pink1B9 mutant adult flies. n = 3 × 3 animals. d, Dietary C18:0 supplementation significantly improves locomotor defects of 2-week-old male Pink1B9 mutant flies. Locomotion quantified as animals climbing up past a threshold in a given amount of time (technical duplicates, biological quadruplicates, ten animals per assay). e, Parkin loss of function in flies leads to mitochondrial fragmentation, which is rescued by dietary supplementation with C18:0. Guts from 14-day-old female control or park25 mutant adult flies expressing mitoGFP and grown on food supplemented with or without C18:0 (10%) were dissected and mitochondria were imaged. Quantification of mitochondrial fragmentation is shown (3 animals per condition, 6 optical areas per animal). b–d, Control flies are the revertant line Pink1RV . Error bars show s.d. Not significant (NS) P ≥ 0.05, *P < 0.05.
