Extended Data Figure 5: Elovl6 mutants have other Mfn loss-of-function phenotypes such as reduced mitochondrial-associated membranes and abnormal cristae.

a, The mitochondrial associated membranes (MAM) band is strongly reduced or absent in Percoll gradients of crude mitochondrial fractions from Elovl6-mutant animals, compared with controls. b, Purity control of mitochondrial preparations show that pure mitochondrial fractions are lacking markers of other subcellular organelles such as calnexin (endoplasmic reticulum) and lamin (nuclei). See Supplementary Fig. 17 for image of the uncropped full western blot. Right, quantification shows that levels of the endoplasmic reticulum marker calnexin are reduced in crude mitochondrial fractions from Elovl6 mutants, compared with controls, in agreement with reduced MAMs in Elovl6 mutants. Values show densitometry ratios of calnexin levels in crude mitochondrial fractions, normalized to total lysate calnexin. c, Electron microscopy of Drosophila S2 cell mitochondria (left) reveals cristae abnormalities in Elovl6-depleted cells. Middle, quantification (n = 200). Significance of the difference was calculated with a Mann–Whitney test (*P < 0.05). Right, average circularity of mitochondria was calculated with ImageJ software. Scale bar, 1 µm. n = 200, ****P < 0.0001, two-tailed t-test. Error bars show s.d.