Extended Data Figure 6: iTRAQ analysis showing the presence of Idh1 and Idh2 precursors in the cytosol of AAC2A128P -expressing cells.
From: A cytosolic network suppressing mitochondria-mediated proteostatic stress and cell death
a, Underlined are Idh1 peptides detected by mass spectrometry. The presequence of Idh1 is shown in red. The cleavage site of mitochondrial peptidase for Idh1 maturation is indicated by the red arrow. Boxed is the peptide 1 detected by mass spectrometry after trypsinization. Peptide 1 encompasses the last threonine residue of the presequence, suggesting that it is derived from the Idh1 precursor instead of its mature form. b, Frequency of Idh1 peptides identified by mass spectrometry. c, Underlined are Idh2 peptides detected by mass spectrometry. The presequences of Idh2 are shown in red. The cleavage sites of mitochondrial peptidase for Idh2 maturation are indicated by the red arrows. Boxed is the peptide 1 detected by mass spectrometry after trypsinization. Peptide 1 encompasses the last 1–2 residues of the presequence, suggesting that it is derived from the Idh2 precursor instead of its mature form. d, Frequency of Idh2 peptides identified by mass spectrometry.
