Extended Data Figure 9: Suppression of ρo-lethality in atp1Δ, mgr2Δ and tom70Δ mutants by overexpression of GIS2 and protein synthesis rate in cells overexpressing GIS2, NOG2 and TMA7.
From: A cytosolic network suppressing mitochondria-mediated proteostatic stress and cell death
a, Ethidium bromide (EB) sensitivity test. Yeast transformants were diluted in water and spotted on YPD with or without ethidium bromide. The plates were incubated at 30 °C for four days. GIS2 was overexpressed from the multicopy vector pRS425. b, In vivo protein synthesis assay. The incorporation of [35S]methionine in the wild-type cells overexpressing GIS2, NOG2 and TMA7 on a multicopy vector was measured at 25 °C.
