Extended Data Figure 2: Mitochondrial damage increases protein aggregation in the cytosol.
From: A cytosolic network suppressing mitochondria-mediated proteostatic stress and cell death
a, Synthetic lethality between mitochondrial damage and cytosolic protein misfolding. Growth of AAC2A128P but not wild-type cells is inhibited by expression of GAL10-HD(25Q) on galactose medium, whereas growth of both AAC2A128P and wild-type cells is inhibited by expression of GAL10-HD(103Q). Yeast transformants were serially diluted in water and spotted on minimal glucose or galactose medium. The plates were incubated at 25 °C for 4 days. b, HD(25Q) forms aggregates in ρo but not ρ+ cells, whereas HD(103Q) is aggregated in both types of cells. The images are representatives of 200 cells examined for each strain. c, Increased cytosolic accumulation and aggregation of green fluorescent protein (GFP)-tagged Aco1 in AAC2A128P -expressing cells. Representative images are from four independent experiments, with a total number of 2,397 and 1,608 cells examined for the wild-type and AAC2A128P strains, respectively. d, Mean ± s.d. of the four experiments in c (P < 0.001, unpaired Student’s t-test).
