Extended Data Figure 5: Increased cytosolic retention and Ssb1 association of mitochondrial precursors in AAC2A128P cells.
From: A cytosolic network suppressing mitochondria-mediated proteostatic stress and cell death
a, Western blot showing increased retention of representative mitochondrial proteins (Aco1, Ssc1, Idh1 and Aac2) in the cytosol of AAC2A128P cells. Pgk1, the cytosolic 3-phosphoglycerate kinase, is used as a control. b, SDS–PAGE (left) and western blot (right) showing Coomassie-stained Ssb1–TAP (tandem affinity purification) pull-down products and increased association of Ssb1–TAP with the mitochondrial Aco1 and Abf2 in AAC2A128P cells, respectively. Full scans of blots and gels are available in the Supplementary Information.
