Extended Data Figure 4: FACS sorting gates for GFP+ cells in Axin2-rtTA;TetO-H2B-GFP mice.
From: Self-renewing diploid Axin2+ cells fuel homeostatic renewal of the liver
Eight-week-old Axin2-rtTA;TetO-H2B-GFP mice were labelled with doxycycline for 7 days and chased for various lengths of time. Hepatocytes were enzymatically dispersed and sorted by FACS. a–c, Successive gating shows sequential selection of all hepatocytes (a), single cells by forward scatter (b), and side scatter (c). d, Dead cells were excluded by propidium iodide labelling. e, GFP-positive cells were gated and either sorted for RNA-seq analysis or further graphed as histograms for GFP intensity analysis (see Fig. 3g).
