Extended Data Figure 5: Axin2 gene dosage and tamoxifen have no effect on pericentral hepatocyte proliferation rate.
From: Self-renewing diploid Axin2+ cells fuel homeostatic renewal of the liver
Wild-type and Axin2CreERT2+/− mice were given EdU daily for 7 days. A subset of wild-type and Axin2CreERT2+/− mice was given 4 mg of tamoxifen per 25 g body weight daily for 5 days. Pericentral hepatocytes were identified by Hnf4a+/GS+ staining. All other hepatocytes were identified by Hnf4a+/GS− antibody staining. The EdU-positive rates within the two hepatocyte populations as a percentage of total HNF4a+ cells were essentially the same regardless of Axin2 gene dosage or tamoxifen administration. n = 5 animals per group. Data represent mean ± s.e.m. *P > 0.05.
