Extended Data Figure 7: FACS sorting gates for GFP+ cells in Axin2-CreERT2;Rosa26-mTmGflox mice for ploidy analysis.
From: Self-renewing diploid Axin2+ cells fuel homeostatic renewal of the liver
Eight-week-old Axin2-CreERT2;Rosa26-mTmGflox mice were labelled with five daily doses of tamoxifen and traced for 7 days. Hepatocytes were enzymatically dispersed and sorted by FACS. a–c, Successive gating show sequential selection of all hepatocytes(a), single cells by forward scatter(b), and side scatter (c). d, Dead cells were excluded by propidium iodide labelling. e, GFP-positive cells were gated and graphed as histograms for Hoechst staining (see Fig. 4).
