Extended Data Figure 6: PINK1 directly stimulates mitophagy after mitochondrial damage.
From: The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy
a, c, Representative FACS data of mt-mKeima-expressing wild-type or PINK1 knockout HeLa cells (a) or PINK1 knockout cells rescued with PINK1 wild-type and then untreated or treated with oligomycin and antimycin A (c). b, d, Average percentage of mitophagy for two replicates of a and c, respectively. e, Representative images of wild-type HeLa cells expressing mCh–OPTN and treated with oligomycin and antimycin A as indicated and immunostained for TOM20 (n = 3). f, Quantification of mCh–OPTN translocation from cells in e. Data are mean ± s.d. from three independent experiments. ***P < 0.001 (one-way ANOVA). NS, not significant.
