Extended Data Figure 8: Analysis of LC3 family members and their translocation to damaged mitochondria in autophagy receptor knockout cell lines.
From: The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy
a, Representative images of wild-type, pentaKO and ATG5 knockout HeLa cells expressing mCh–parkin and GFP–LC3B were immunostained for TOM20 (n = 3). b, Cell lysates from mCh–parkin expressing wild-type, pentaKO and ATG5 knockout cells were immunoblotted. c, Representative images of wild-type, NDP52/OPTN DKO and pentaKO cells expressing mCh–parkin and GFP-tagged LC3A, LC3B or LC3C were immunostained for TOM20 (n = 3; see Fig. 4a for quantification). d, e, Representative images of wild-type and NDP52/OPTN/TAX1BP1 triple knockout cells expressing mCh–parkin and GFP–LC3C were immunostained for TOM20 (n = 3) (d) and quantified for GFP–LC3C translocation to mitochondria (e). Data in e are mean ± s.d. from three independent experiments. ***P < 0.001 (one-way ANOVA). Scale bars, 10 μm.
