Extended Data Figure 9: GABARAP proteins do not translocate to damaged mitochondria, and early stages of autophagosome biogenesis mediated by WIPI1 and DFCP1 are inhibited in autophagy-receptor-deficient cell lines.
From: The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy
a–c, Representative images of wild-type, NDP52/OPTN DKO and pentaKO cells expressing mCh–parkin and GFP-tagged GABARAP, GABARAPL1 or GABARAPL2 (a), GFP–WIPI1 (b) or GFP–DFCP1 (c) immunostained for TOM20 (n = 3 for each condition, see Fig. 4b and c for quantification of b and c). d, mCh–parkin cell lines as indicated were subjected to either phos-tag SDS–PAGE or standard SDS–PAGE followed by immunoblotting. Arrows indicate the position of phosphorylated beclin species. e, Representative images of untreated wild-type, NDP52/OPTN DKO and pentaKO cell lines expressing mCh–parkin and GFP–ULK1 were immunostained for TOM20 and GFP (n = 3 experiments). Scale bars, 10 μm.
