Extended Data Figure 5: PINK1 directly stimulates mitophagy in the absence of mitochondrial damage.
From: The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy
a, b, Cells were treated with rapalog and analysed by FACS for lysosomal-positive mt-mKeima. Representative data for wild-type HeLa (a) and pentaKO cells (b) without or with Flag/HA–OPTN. c, d, Cell lysates from pentaKO cells expressing FRB-Fis1, PINK1Δ110–YFP–2×FKBP, mt-mKeima and wild-type Flag/HA–OPTN (c) or mutants, (d) Flag/HA–p62, wild-type Flag/HA–NDP52 or NDP52 mutants as indicated were assessed for receptor expression by immunoblotting. e, f, Cells from c and d were treated with rapalog and analysed by FACS for lysosomal-positive mt-mKeima. Representative data of two experiments are presented. g, Cell lysates from pentaKO cells expressing FRB-Fis1, with or without Flag/HA–OPTN and wild-type or kinase-dead PINK1Δ110–YFP–2×FKBP were assessed for OPTN by immunoblotting. h, Flag/HA–OPTN pentaKO cells expressing FRB-Fis1, PINK1Δ110–YFP–2×FKBP, mt-mKeima-transfected and either vector or untagged parkin were analysed by FACS. Representative data of two experiments are presented.
