Extended Data Figure 6: SC18 staining of ferret respiratory tissues.
From: The soft palate is an important site of adaptation for transmissible influenza viruses
a–d, f, g, Sections of ferret trachea (a), lung (b), soft palate (c, d), biopsy of nasal turbinate (NT) tissue with respiratory epithelium (RE) (f) and olfactory epithelium (OLF) (g) were stained with purified SC18 HA protein to identify areas expressing long-chain α2,6-linked sialic acids. h, Illustration of ferret head (sectioned along the midline) highlighting the anatomical locations of respiratory epithelium and OLF tissues. Goblet cells on the respiratory epithelium of the soft palate (nasopharyngeal surface) also stained positive for SC18 (d). e, Absence of SC18 staining after sialidase A treatment indicates the high specificity of SC18 for the respiratory epithelium of the soft palate. All scale bars are 100 μm unless indicated.
