Extended Data Figure 8: Characterization of non-epithelial structures in the kidney organoid.
From: Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis
All images were taken from day 18 kidney organoids. a, PDGFRA+ pericytic cells attaching on KDR+ vessels. Scale bar, 50 μm. b, Some glomeruli contained PDGFRA+ cells likely to represent early mesangial cells19. Scale bar, 50 μm. c, Laminin staining (LAM) demonstrates the presence of basement membrane in glomerulus structures (white arrowheads). Scale bar, 100 μm. d, TEM images of avascular glomeruli showing early podocytes surrounding a basement membrane (yellow arrowheads) and exhibiting foot processes on the basement membrane. e, Immunofluorescence showing FOXD1 expression in podocytes (WT1+FOXD1+)18 and a subpopulation of MEIS1+ interstitium (white arrowheads). This is suggestive of the presence of both cortical stroma (FOXD1+MEIS1+) and medullary stroma (FOXD1−MEIS1+). Scale bar, 100 μm.
