Extended Data Figure 6: Clonal growth rates do not change in the absence of Dpp spreading.
From: Dpp spreading is required for medial but not for lateral wing disc growth
a–h, Estimation of clonal proliferation rates as shown in Fig. 4, inducing Raeppli at different time points: either 20 h before dissection (a–d) or 41 h before dissection (e–h). Discs were dissected at 96–100 h AEL. a, e, Representative control discs. b, f, Representative discs with blocked Dpp spreading. c, g, Clone size (number of cells per clone) plotted against the relative position in the posterior compartment (0 corresponding to the A/P boundary and 1 to the posterior edge of the disc). Low numbers of small clones in proximity to the A/P boundary are found in discs with blocked Dpp spreading (red dots), while these small clones are not present in control discs (black dots; see also Extended Data Fig. 7). d, h, Boxplots showing the number of cells per clone. When the small clones are excluded (right boxplots) no significant differences are detected in clonal proliferation between control discs and discs with blocked Dpp spreading (P > 0.05).
