Extended Data Figure 4: Time course of eGFP::Dpp spreading, signalling and the mitotic index.
From: Dpp spreading is required for medial but not for lateral wing disc growth
a–i, Time course of extracellular eGFP::Dpp (exGFP), Dpp signalling (p-Mad) and p-H3 from 64–112 h AEL of larval development. a, b, Representative discs of the six time points examined of control animals (a) and animals with blocked Dpp spreading (b) stained for exGFP. The region marked by a red rectangle is enlarged below each image. eGFP::Dpp spreading is tightly blocked by morphotrap at all time points. c, Average exGFP profiles for all time points (control in black/block in red: n = 43/29). d, e, Discs of control animals (d) and animals with blocked Dpp spreading (e) stained for p-Mad. When Dpp spreading is blocked, the p-Mad gradient also collapses onto the source region at all time points. f, Average p-Mad profiles (control/block: n = 50/35). g, h, Control discs (g) and discs with blocked Dpp spreading (h) stained for p-H3. i, Quantification of the mitotic index (p-H3 spot density). No significant differences were observed between control discs (black, n = 55) and discs with blocked Dpp spreading (red, n = 43) at any time point (n > 0.05 for all time points, two-sided t-test, unequal variance).
