Extended Data Figure 1: Induction of MsrPQ by HOCl is dependent on the presence of a functional YedVW two-component system.
From: Repairing oxidized proteins in the bacterial envelope using respiratory chain electrons
Top, immunoblot analysis shows that the induction of MsrP synthesis by HOCl (0.2 mM) is yedW-dependent. The image is representative of experiments made in biological triplicate. Bottom, an msrP::lacZ fusion was used as a read-out for msrP expression. Deletion of yedV upregulates msrP expression, while deletion of yedW prevents its induction by HOCl. Error bars, mean ± s.e.m.; n = 4. The uncropped blot is shown in Supplementary Fig. 4.
