Extended Data Figure 4: Preparation of pure diastereoisomeric forms of Met-O.
From: Repairing oxidized proteins in the bacterial envelope using respiratory chain electrons
a, The Oak Ridge Thermal Ellipsoid Plot (ORTEP ellipsoid) representation with 50% probability level of the crystal structure for the isolated salt of l-methionine-S-sulfoxide (right) picrate (left). The grey, blue, red, white and yellow spheres respectively represent carbon, nitrogen, oxygen, hydrogen and sulfur atoms. b, Chemdraw representation of l-methionine-R,S-sulfoxide with proton and carbon positioning (relative to NMR assignment). c, Zoom on the 1H NMR spectra of ~150 mM solutions of l-methionine sulfoxide in D2O pD 6.5, either as a mixture of R- and S- diastereoisomers (top), isolated S- (middle) or isolated R- (bottom) (containing 30 mM dioxane as an internal reference). d, Zoom on the 13C NMR spectra of ~150 mM solutions of l-methionine sulfoxide in D2O pD 6.5, either as a mixture of R- and S- diastereoisomers (top), isolated S- (middle) or isolated R- (bottom) (containing 30 mM dioxane as an internal reference).
