Extended Data Figure 5: The whole cell currents and G/V curves of AtTPC1 with mutations at the luminal Ba2+ binding sites.
From: Structure of the voltage-gated two-pore channel TPC1 from Arabidopsis thaliana
a–d, D454N (a), D240N (b), E528Q (c) and E239Q (d). The bath solutions contained 0, 0.1, 1, or 10 mM [Ca2+]extracellular. The pipette solutions contained 300 μM [Ca2+]cytosolic. Data measured in 0.1 mM [Ca2+]extracellular are shown in the main text Fig. 4e.
