Extended Data Figure 6: Functional analysis of AtTPC1 mutants.
From: Structure of the voltage-gated two-pore channel TPC1 from Arabidopsis thaliana
a, The whole-cell currents of AtTPC1 containing EF-hand Ca2+-site mutations (D335A in EF1 and D376A in EF2). Currents were recorded with the presence of 300 μM [Ca2+]cytosolic. b, Whole-cell currents and G/V curves of AtTPC1 with neutralization mutations of arginines on IS4 and IIS4 of the voltage-sensing domains.
