Extended Data Figure 9: Predicting cell division based on TCJ distribution.
From: Epithelial tricellular junctions act as interphase cell shape sensors to orient mitosis
a, a′′, Rose plots of the magnitude of the difference between experimental (
) and predicted division orientations by the average (60–30 min before mitosis) interphase TCJ bipolarity (
) or cell long axis (
) in cells for the indicated 
intervals. To facilitate the comparison between the left and the right rose plots, the data are duplicated relative to 0° line (light blue and light red). Kolmogorov–Smirnov test (P values), percentage of total cells (n = 29,388). Panels b and b′′ are identical to panel e in Fig. 3. b, b′′, Rose plots of the magnitude of the difference between experimental (
) and predicted division orientations by the average (60–30 min before mitosis) interphase TCJ bipolarity (
) or cell long axis (
) for the indicated 
intervals. To facilitate the comparison between the left and the right rose plots, the data are duplicated relative to 0° line (light blue and light red). Kolmogorov–Smirnov test (P values), percentage of total cells (n = 29,388). Panels c and c′′ are identical to panel f in Fig. 3. c, Plot of the spindle orientation prediction improvements (colour-coded from dark blue to red) based on TCJ bipolarity over those based on cell shape versus the magnitude of their angular difference (
) and the cell shape anisotropy (
). The plot height is the normalized cell number in each domain of the plot (29,883 cells were analysed in total). As 
increases, the TCJ bipolarity predictions improve over cell shape prediction for both rounded (low 
) and elongated cells (high 
). Whereas the rounded cells are characterized by an even distribution along the 
axis, the elongated cells are mainly characterized by a strongly skewed distribution towards low 
.
