Extended Data Figure 3: Regulation of Dlg, Gli and Mud localization in epithelial tissue.
From: Epithelial tricellular junctions act as interphase cell shape sensors to orient mitosis
a, b, Localizations of Gli (white in a and green in a′, n = 2 clones) and Dlg (white in b and green in b′, n = 3 clones) in fixed notum tissues harbouring mud clones (identified by loss of mRFP–nls, red in a′–b′). The loss of Mud function does not modify the Gli and Dlg localizations at septate junctions. c, Localization of Gli–YFP (white in c and green in c′) in live notum tissue harbouring a clone of dlg (identified by the loss of mRFP–nls, red in c′, n = 13 clones). The loss of Dlg function results in a loss of Gli localization at TCJs. d, Localization of Dlg–GFP (white in d and green in d′) in live epithelial dorsal thorax tissue harbouring a Gli clone (identified by expression of PH–ChFP, red in d′, n = 5 clones). The loss of Gli function does not affect the distribution of Dlg–GFP at the septate junctions. Scale bars, 5 μm.
