Extended Data Figure 5: Upregulation of AxMlp transcript during early regeneration and alteration of AxMLP protein localization in wound epidermis cells.

a, e, Measurement of AxMlp expression by qPCR at the indicated time points during tail (a) and limb (e) regeneration (n = 3: biological replicates; mean ± s.d.). To obtain the values of fold-change for each time point, the relative concentrations of the PCR products were calculated by the standard curve method. The concentration of AxMlp was normalized to that of large ribosomal protein 4 (Rpl4). b–h, Immunostaining with anti-AxMLP antibody (white) on tails (b–d) and limbs (f–h) of intact (b, f: transverse sections), 1 dpa (c: sagittal; g: horizontal) and 6 dpa (d: sagittal; h: horizontal) samples. By 6 dpa, the epithelial organization and AxMLP expression appeared to be returning to a less tightly adherent, less membrane-associated appearance (d, h). Scale bars: left, 200 μm; right 50 μm. Red arrowheads indicate spinal cord; green arrowheads indicate wound epidermis; yellow arrowheads indicate normal epidermis; yellow circles indicate two bones in the lower limb.