Extended Data Figure 5: Hypoxia and anchorage independence elicit different effects on citrate metabolism.
From: Reductive carboxylation supports redox homeostasis during anchorage-independent growth
a, Mass isotopologues of citrate and malate in H460 cells cultured with [U-13C]glutamine, in monolayer or spheroid conditions, under 21% or 1% oxygen (n = 4 cultures from two experiments). b–d, Contribution of glutamine (b), glucose (c) and acetate (d) to the lipogenic acetyl-CoA pool used for palmitate synthesis, during 24 h of culture with each tracer (n = 3 cultures in panels b and c; n = 2 cultures in panel d from a representative experiment). All data represent mean ± s.d. *P < 0.05, Welch’s unequal variances t-test followed by multiple-comparison correction (a), or ANOVA (b), or Welch’s unequal variances t-test (c). The experiment in d was repeated twice, and all other experiments were repeated 3 times or more.
