Extended Data Figure 9: Pan-neuronal NAc recordings: increased activity associated with risky decisions.
From: Nucleus accumbens D2R cells signal prior outcomes and control risky decision-making
a, Median-normalized dF/F signal during the first second of the outcome period for each hSYN-GCaMP6m-expressing rat, comparing risky outcomes to safe outcomes (n = 8; Wilcoxon matched-pairs test, W = 36, P = 0.008). b, Lack of correlation between the proportion of choices made by each rat to the risky lever and the individual’s risky versus safe outcome signal ((dF/F) during the first 1 s of risky outcome/(dF/F) during safe outcome) (n = 8 rats, Pearson’s r2 = 0.12, P = 0.40). c, Median-normalized dF/F signal during the first second of the outcome period for each D2SP-GCaMP6m-expressing rat, comparing safe outcomes to risky outcomes (n = 6; Wilcoxon matched-pairs test, W = 17, P = 0.04). d, Lack of correlation between the proportion of choices made by each rat to the risky lever and the individual’s risky versus safe outcome signal ((dF/F) during the first 1 s of safe outcome/(dF/F) during risky outcome) (n = 6; Pearson’s r2 = 0.11, P = 0.51). e, Median-normalized dF/F signal at the time of trial initiation for each hSYN-GCaMP6m-expressing rat, sorted on previous trial outcome, comparing risky outcomes to safe outcomes (n = 8; paired t-test, t7 = 7.25, P = 0.0002). f, Lack of correlation between the proportion of choices made by each rat to the risky lever and the individual’s risk signal ((dF/F) at nosepoke trial initiation after risky outcome/(dF/F) after safe outcome) (n = 8; Pearson’s r2 = 0.01, P = 0.78). g, Median-normalized dF/F signal at the time of trial initiation for each D2SP-GCaMP6m-expressing rat, sorted on previous trial outcome, comparing risky outcomes to safe outcomes (n = 6; paired t-test, t5 = 6.901, P = 0.001). h, Correlation between the proportion of choices made by each D2SP-GCaMP6m-expressing rat to the risky lever and the individual’s risk signal ((dF/F) at nosepoke trial initiation after risky outcome/(dF/F) after safe outcome) (n = 6; Pearson’s r2 = 0.97, P = 0.0003). i, Median-normalized dF/F signal at the time of trial initiation for each hSYN-GCaMP6m-expressing rat, sorted on upcoming choice, comparing risky choices to safe choices (n = 8; paired t-test, t7 = 2.11, P = 0.036). j, Lack of correlation between the proportion of choices made by each rat to the risky lever and the individual’s decision period signal ((dF/F) at nosepoke trial initiation before a risky choice/(dF/F) before a safe choice) (n = 8; Pearson’s r2 = 0.17, P = 0.31). k, Median-normalized dF/F signal at the time of trial initiation for each D2SP-GCaMP6m-expressing rat, sorted on upcoming choice, comparing risky choices to safe choices (n = 8; paired t-test, t7 = 2.11, P = 0.036). l, Lack of correlation between the proportion of choices made by each rat to the risky lever and the individual’s safe choice signal ((dF/F) at nosepoke trial initiation before choosing safe/(dF/F) at nosepoke before choosing risky) (n = 6; Pearson’s r2 = 0.12, P = 0.48). Data from k and l also appear in Fig. 3i, o and are reproduced here for ease of comparison. All error bars represent s.e.m.
