Extended Data Figure 7: Regulation of p-mTOR and mTORC1 signalling by c-Myc.
From: Metabolic maintenance of cell asymmetry following division in activated T lymphocytes
a, b, Quantification of p-mTOR staining and c-Myc–GFP for OT-I CD8 T cells co-cultured with SIINFEKL-pulsed BMDCs 80.9% both bright in proximal daughter (x2 = 25.67, DF = 3, P < 0.0001 chi-square goodness of fit test); R2 = 0.2307, P = 0.0275, linear regression (a); or T cells stimulated for 36 h with anti-CD3, anti-CD28, and ICAM 82.4% concordance of markers (P = 0.0013, two-tailed binomial test); R2 = 0.1204, P = 0.1725, linear regression (b). Asymmetry as assessed by fluorescence intensity is expressed as (proximal − distal)/total (a), or values from (c-Mychigh – c-Myclow)/total (b). c, Western blot analysis of CD8 T cells activated with anti-CD3, anti-CD28, and ICAM for 6 h without treatment (ctrl) or with 1 μM or 5 μM of the bromodomain inhibitor JQ1. Data are representative of three independent experiments.
