Extended Data Figure 8: Asymmetric assortment of mTORC1 activity with c-Myc–GFP.
From: Metabolic maintenance of cell asymmetry following division in activated T lymphocytes
a, b, Representative confocal images for quantifications of c-Myc–GFP and pS6 staining corresponding to Fig. 3a, b (a) or c-Myc–GFP and p70S6K corresponding to Fig. 3c (b) in OT-I T cells co-cultured with SIINFEKL-pulsed BMDCs. c, d, Quantifications for c-Myc–GFP and pS6 100% both bright in proximal daughter (x2 = 19.89, DF = 3, P = 0.0002, chi-square goodness of fit test); R2 = 0.9457, P = 0.0055, linear regression (c); or p70S6K 100% both bright in proximal daughter (x2 = 14.14, DF = 3, P = 0.0027, chi-square goodness of fit test); R2 = 0.4875, P = 0.0026, linear regression (d) staining in T cells stimulated on anti-CD3, anti-CD28, and ICAM for 36 h. e, Representative confocal images for quantifications of c-Myc–GFP and pFOXO1 staining corresponding to Fig. 3d, e. T cells stimulated with anti-CD3, anti-CD28, and ICAM for 36 h.
