Extended Data Figure 9: First-division CD8^high c-Myc–GFP^high CD8 T cells are more glycolytic and exhibit more glutaminolysis and pentose phosphate pathway activity than first division CD8^low c-Myc–GFP^low CD8 T cells but have decreased FAO.

Undivided or first-division antibody-activated T cells were sorted on c-Myc expression. 1.6 million T cells from each group were analysed via UHPLC/MS/MS for unbiased metabolomic profiling performed by Metabolon, Inc. a–d, Metabolic pathway schematic (a) was generated by Metabolon Inc., as were graphs (b) for select metabolites in pathway, representing data for DNA-normalized data. Metabolic pathway schematics and graphs were also generated by Metabolon Inc. for select metabolites in the pathway, representing data for DNA-normalized data for nucelotide biosynthesis (c) and fatty acid oxidation (d).