Extended Data Figure 10: c-Myc^high CD8 T cells are more glycolytic than CD8^low CD8 T cells without asymmetric distribution of mitochondria.

a, The extracellular acidification rate (ECAR) for sorted c-Myc^high (circles) and c-Myc^low (squares) T cells from the first division after 36 h of activation with anti-CD3, anti-CD28, and ICAM as measured by a Seahorse Bioflux analyser during exposure to the indicated compounds are represented. Values are paired across three independent experiments. b, The oxygen consumption rate (top panels) and ECAR (bottom panels) for basal respiration in complete RPMI with glucose of sorted c-Myc^high (shaded bars) and c-Myc^low (open bars) CD8 T cells from the first-division after 36 h of activation by anti-CD3, anti-CD28, and ICAM, as measured by a Seahorse Bioflux analyser across three independent experiments. c, Quantification of mitochondrial DNA in sorted first-division c-Myc low and high OT-I CD8 T cells activated on anti-CD3, anti-CD28 and ICAM for 36 h. Mean + s.d. are plotted for three technical replicates of n = 2 mice per group. d, Overlay and individual channel images of β-tubulin (blue), DAPI (grey), c-Myc–GFP (green), and TOM20 (red) of an OT-I CD8 T cell activated with anti-CD3, anti-CD28 and ICAM for 36 h.