Extended Data Figure 7: Transcription coupled-repair is impaired at active TFBS.
From: Nucleotide excision repair is impaired by binding of transcription factors to DNA
To carry out this analysis, TFBS overlapping transcribed regions (located 200–500 bp downstream of TSS) were centred at the TFBS mid-point. We plotted the mutation and repair rates of UV induced damages (CPD and 6–4PP) in XP-C cells, which possess only transcription-coupled repair capability. TFBS in either strand were separated: those in the template strand of the gene are shown in the left panel, while those in the non-template strand are presented in the right panel. All TFBS and their flanking regions are shown in the same orientation (5′ to 3′). This result shows that TF binding to both strands results in lower transcription-coupled NER activity.
