Extended Data Figure 6: Phylogeny of fizzy family proteins and effects of expressing cortex in the Drosophila wing.
From: The gene cortex controls mimicry and crypsis in butterflies and moths
a, Neighbour joining phylogeny of fizzy family proteins including functionally characterized proteins (in bold) from Saccharomyces cerevisiae, Homo sapiens and D. melanogaster as well as copies from the basal metazoan Trichoplax adhaerens and a range of annotated arthropod genomes (Daphnia pulex, Acyrthosiphon pisum, Pediculus humanus, Apis mellifera, Nasonia vitripennis, Anopheles gambiae and Tribolium castaneum) including the lepidoptera H. melpomene (in blue), D. plexippus and B. mori. Branch colours: dark blue, cdc20/fzy; light blue, rap; red, lepidopteran cortex. b–e, Ectopic expression of cortex in D. melanogaster. Drosophila cortex produces an irregular microchaete phenotype when expressed in the posterior compartment of the fly wing (c) whereas Heliconius cortex does not (d), when compared to no expression (b). A, anterior; P, posterior. Successful Heliconius cortex expression was confirmed by anti-HA immunohistochemistry in the last instar Drosophila larva wing imaginal disc (e, red), with DAPI staining in blue.
