Extended Data Figure 5: Alternative splicing of cortex.
From: The gene cortex controls mimicry and crypsis in butterflies and moths
a, Amplification of the whole cortex coding region, showing the diversity of isoforms and variation between individuals. b, Differences in splicing of exon 3 between H. melpomene aglaope and H. melpomene amaryllis. Products amplified with a primer spanning the exon 2–4 junction at three developmental stages. The lower panel shows verification of this assay by amplification between exons 2 and 4 for the same final instar larval samples (replicated in Extended Data Fig. 2c). c, Lack of consistent differences between H. melpomene melpomene and H. melpomene rosina in splicing of exon 3. Top panel shows products amplified with a primer spanning the exon 2–4 junction; lower panel shows the same samples amplified between exons 2 and 4. d, Differences in splicing of exon 5 between H. melpomene melpomene and H. melpomene rosina. Products amplified with a primer spanning the exon 4–6 junction at three developmental stages. e, Subset of samples from d amplified with primers between exons 4 and 6 for verification (middle, 24-h pupae samples are replicated in Extended Data Fig. 2d). f, Lack of consistent differences between H. melpomene aglaope and H. melpomene amaryllis in splicing of exon 5. Products amplified with a primer spanning the exon 4–6 junction. g, H. melpomene cythera also expresses the isoform lacking exon 5, while a pool of six H. melpomene malleti individuals do not. h, Expression of the isoform lacking exon 5 from an F2 H. melpomene melpomene × H. melpomene rosina cross. Individuals homozygous or heterozygous for the H. melpomene rosina HmYb allele express the isoform while those homozygous for the H. melpomene melpomene HmYb allele do not. i, Allele-specific expression of isoforms with and without exon 5. Heterozygous individuals (indicated with blue and red stars) express only the H. melpomene rosina allele in the isoform lacking exon 5 (G at highlighted position), while they express both alleles in the isoform containing exon 5 (G/A at this position).
